Cascade II:1024 The Most Versatile Megapixel EMCCD Camera0 pages

Cascade® II 1024 Datasheet
HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES

1024 x 1024 imaging array
13 x 13-μm pixels

Primary applications
Low-light fluorescence
Total internal reflection fluorescence (TIRF)
Single-molecule fluorescence
Intracellular trafficking studies

The Photometrics® Cascade® II 1024 is the
only true 16-bit, megapixel EMCCD camera
in the world to offer -60˚C cooling without
LN2 or water! This high-resolution camera
uses its deep thermoelectric cooling to
maximize gain and minimize dark current.
A stainless-steel vacuum chamber (with
all-metal seals) houses a high-QE,
back-illuminated, frame-transfer EMCCD.
The camera’s exclusive vacuum technology
is so robust it carries a lifetime guarantee.

FeaturestBenefits
EM gain

Low-noise, impact-ionization process provides very high sensitivity

Back-illuminated EMCCD

Highest available quantum efficiency (>90% peak QE)

Deep cooling

Thermoelectric cooling to -60°C minimizes dark current and allows long exposure times
No need for a bulky chilled-water circulator or cryogenic compressor, both of which are prone to leaks, blockages, and
condensation

Lifetime vacuum

Permanent, all-metal vacuum seals guaranteed for lifetime of camera
Maintenance-free operation

1024 x 1024 imaging array
13 x 13-μm pixels

Good field of view and sensitivity
Good resolution

10-MHz readout
5- and 1-MHz readout

Excellent for high-speed image visualization
Perfect for high-precision photometry

Dual amplifiers

Select readout mode via software:
(1) optimal high-speed / high-sensitivity performance
(2) optimal wide-dynamic-range performance

16-bit digitization

Wide dynamic range allows detection of bright and dim signals in the same image

Frame-transfer EMCCD

100% duty cycle for continuous data collection
No mechanical shutter required

Single optical window

Single vacuum window is the only optical surface between incident light and EMCCD surface
No light loss from multiple optical surfaces

C-mount

Easily attaches to microscopes, standard lenses, or optical equipment

Acquisition software

Captures, analyzes, and saves high-resolution images

PCI interface

High-bandwidth, uninterrupted data transfer

PVCAM
Circular buffers
Device sequencing

Supported by numerous third-party software packages
Real-time focus
Precise integration with shutters, filter wheels, etc.

®

Compatible with Windows® XP/7

PM-DS-005 Rev B3

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