QuantEM:512SC Full Field-of-View Fluorescence Microscopy0 pages

QuantEM™ 512SC Datasheet
HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES

512 x 512 imaging array
16 x 16-μm pixels
The Photometrics® QuantEM™ 512SC is the world’s first and only
electron-multiplying CCD (EMCCD) camera to offer on-chip
signal amplification with true quantitative stability across 16 bits at
readout rates of 10, 5, and 1.25 MHz. The high-speed, highperformance QuantEM lets you conduct precision ratiometric analysis
in time-course experiments, acquire reproducible data during long-term
studies, and capture streaming data for multidimensional time-lapse
investigations — all with single-molecule sensitivity. A patent-pending
PAR™ feedback system provides exceptional stabilization of EM gain,
while an intelligent FPGA design facilitates self-calibrating linearization
of EM gain and prevents bias drift over time. Furthermore, patentpending ACE™ technology enables superior timing resolution of the
device’s pixel clocks, allowing optimal signal-to-noise sampling and
minimizing spurious charge.

Primary applications
Quantitative FRET
Multiprobe experiments
Ratiometric ion imaging
Confocal microscopy
Live-cell fluorescence imaging

FeaturestBenefits
EM gain

Very high sensitivity
Low-noise, impact-ionization process

Back-illuminated EMCCD

Highest available quantum efficiency (>90% peak QE)

512 x 512 imaging array
16 x 16-μm pixels

Good field of view and sensitivity
Good resolution

Intelligent FPGA design

Precise linearization of EM gain
Self-calibrating linearization ensures truly quantitative data all the time
Prevents bias drift over time to guarantee a stable background

PAR* feedback system
(Photometrics Active Regulation)

Delivers unsurpassed EM gain stability for outstanding signal fidelity across 16 bits

ACE* technology
(Advanced Clocking Enhancement)

Pixel-clock timing resolution 12x better than competing EMCCD cameras
Provides lowest noise floor and minimizes generation of spurious charge and background events

10-MHz readout

Excellent for high-speed image visualization

5- and 1.25-MHz readout

Perfect for high-precision photometry

Dual amplifiers

Select readout mode via software for optimized (1) high-speed / high-sensitivity performance
or (2) wide-dynamic-range performance

16-bit digitization

Wide dynamic range allows detection of bright and dim signals in the same image

Frame-transfer EMCCD

100% duty cycle to collect continuous data
No mechanical shutter required

C- mount

Easily attaches to microscopes, standard lenses, or optical equipment

Turbo-1394™ interface
(IEEE-1394a)

High-bandwidth, uninterrupted data transfer with no dropped frames
Windows® XP/7 compatibility

PVCam®
Circular buffers
Device sequencing

Supported by numerous third-party software packages
Real-time focus
Precise integration with shutters, filter wheels, etc.

*tPatent-pending Photometrics technology
PM-DS-001 Rev C4

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