TEMPRO - Fluorescence Lifetime0 pages

TemPro
Fluorescence lifetime system

Fluorescence lifetime spectroscopy for every laboratory
TemPro brings the power of lifetime spectrofluorometry to researchers who previously found it unaffordable. Recording
fluorescence lifetimes gives you a dynamic picture of your sample’s characteristics, including molecular motion and size,
solvation environment, and intermolecular distances—things that steady-state fluorescence cannot provide. Lifetime
spectrofluorometry is unique in its sensitivity and selectivity: for example, you can temporally separate similar fluorescence
spectra from two distinct species in a solution. TemPro uses Time-Correlated Single-Photon Counting (TCSPC)—the
reliable, intuitive, and most sensitive lifetime-measurement technique. The filter-based TemPro can measure a complete
range of lifetimes from only 100 picoseconds to 1 second, with our NanoLED and SpectraLED solid-state pulsed excitation
sources spanning wavelengths from the UV to NIR. The TemPro lifetime spectrofluorometer is so affordable that any lab now can
exploit the power of fluorescence dynamics coupled with a phosphorescence capability.

Fluorescence decay of 10–5 M
aqueous Erythrosin B, taken
with the TemPro, giving a
lifetime of 85 ps.
Excitation = 488 nm.
Emission > 550 nm.
The green line is the monoexponential fit to the decay data
(red dots) after reconvolution
with the instrumental response
(blue dots).

APPLICATIONS
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FRET (Förster Resonance Energy Transfer)
Stern-Volmer quenching
Efficiency of photosynthesis
Lanthanide luminescence
Fluorescence anisotropy
Protein fluorescence
Phosphorescence lifetimes and anisotropy

Anisotropy decay of 10–5 M
DPH in Kaydol®, recorded
with the TemPro.
Excitation = 373 nm.
Emission > 418 nm.
The initial anisotropy of
0.33 drops to zero showing
a free rotation with 4.9 ns
correlation time.